GC/MS Analysis

The Bergman lab has been performing GC/MS analysis for ongoing research projects as well as collaborative projects since 2004. They analyze plasma samples for concentration and enrichment of:
– Glucose
– Glycerol
– Free fatty acids

Our Methods:
o Glucose and glycerol: Penta-acetate derivatization in EI mode
o FFAs: Fatty acid methyl esters (FAME) in EI mode or PTFB derivatization in CI mode

* This is the typical methodology used but other options are available. Please contact us for further information.

Sample Dropoff:
Option A) Plasma processing and GCMS run: Deliver your plasma and our technicians will extract and derivatize the samples (more expensive).

Option B) GCMS run only: Using our internal standards and chemicals, you can extract and derivatize your own plasma samples in our laboratory (less expensive).

All samples need to be delivered or shipped in a -80C freezer box in the order they are listed on the Sample Inventory Form. Please label each tube with a concise yet unique code (ex. 6428-1A).

All academic analyses are subsidized by the Colorado NORC. Reduced pricing is available for junior investigators. Turnaround time is dependent on the number and types of samples to prepare and run, as well as the amount of samples in our queue. We frequently run hundreds of samples for investigators and the instrument can be tied up for some time so please plan accordingly.
For current pricing and to discuss sample analysis and turnaround time, please contact: Bryan.Bergman@ucdenver.edu

Our Instrument:
Agilent 6890 GC/5973 MS