Assays provided by NORC Lipidomics Core

• Triacylglycerols (TAGs) 
• DAGs/Ceramides/Glycosphingolipids – diacylglycerols (DAGs), ceramides (Cer), dihydroceramides (dHCer), glucosyl ceramides (GluCer), galactosyl ceramides (GalCer), and lactosyl ceramides (LacCer)
• Sphingolipids and Acyl Carnitines – sphingosine (SPH), ceramide-1-phosphate (CerP), acyl carnitines (AC), sphingomyelin (SPM)
• Glycerophospholipids – includes phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidic acid (PA), phosphatidylglycerol (PG), cardiolipin (CL) and all lyso derivatives of these compounds
• Eicosanoids – HETEs, HODEs, leukotrienes, prostaglandins, thromboxane

Sample preparation 

• Option A – samples are homogenized, BCA assay performed, and extracted for lipid analysis (most expensive option)
• Option B – customer prepares samples in NORC Lipidomics laboratory.  Customer is responsible for homogenization, BCA assay, and lipid extraction using the supplies available in the NORC Lipidomics Laboratory
• Option C – customer prepares samples in their lab.  In order to use this option, customers need to be trained by NORC Lipidomics Laboratory staff, obtain the internal standards from the NORC Lipidomics Laboratory, and purchase solvents and glassware approved by the NORC Lipidomics Laboratory (least expensive option)

Sample Delivery

All samples need to be delivered or shipped in a -80C freezer box in the order they are listed on the Sample Inventory Form.  Sample tubes MUST be numbered sequentially.  Any unique code for your samples can be recorded on the Sample Inventory Form in the sample description column.

Pricing

All academic analyses are subsidized by the Colorado NORC. Reduced pricing is available for junior investigators and preliminary data for grant submissions. Quantitative lipidomics is difficult because we look at each and every peak to ensure that we are quantifying a real lipid that is above the limit of detection for each species. Running samples is easy, it is the data analysis that is difficult and requires time and expertise. The cost for samples is proportional to the amount of time it takes to quantify them.  Turnaround time is dependent on the number and types of lipids to analyze, and the number of samples in our queue.  For current pricing and to discuss sample analysis and turnaround time, please contact:  karin.zemskiberry@cuanschutz.edu

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