Molecular and Cellular Analytical Core Team

Bryan Bergman
Director, Molecular and Cellular Analytical Core

Dr. Bergman is a Professor in the Division of Endocrinology, Diabetes, and Metabolism at the University of Colorado Anschutz Medical Campus. Dr. Bergman’s research investigates the relationship between muscle lipids and insulin sensitivity, and he has been continuously funded by the NIH since 2005. His laboratory focuses on two main research themes. One theme seeks to understand the relationship between skeletal muscle subcellular lipid localization and insulin resistance in humans. Specifically, his lab is investigating how the intracellular location, molecular species, and isomers of diacylglycerol and sphingolipids promote insulin resistance in humans. The current study is investigating these changes after insulin sensitizing lifestyle interventions and is funded by R01DK111559. The second research emphasis aims to elucidate how intermuscular adipose tissue impacts skeletal muscle insulin sensitivity, muscle strength, and size in humans. This study combines muscle biopsy visits with elective surgeries to obtain intermuscular, subcutaneous, and visceral adipose tissue biopsies and is funded by R01DK118149. Dr. Bergman’s laboratory also pursues mechanistic relationships between inter- and intra-muscular lipids and insulin sensitivity using primary muscle cell culture. This model is unique as the phenotype of donor is maintained in culture. They are using this model to determine mechanisms underlying the relationship between localized muscle lipids and insulin sensitivity, and how the secretome of intermuscular adipose tissue promotes insulin resistance. The overall goal of Dr. Bergman’s research is to uncover novel therapeutic targets to increase muscle insulin sensitivity, a need not bet by currently therapies, to help prevent and treat pre-diabetes and type 2 diabetes.

Molecular Core
Michael Rudolph, P.h.D.
Associate Director, Molecular and Cellular Analytical Core
Dr. Rudolph is Assistant Professor in the Division of Endocrinology, Metabolism, and Diabetes, where he investigates how maternal derived fatty acids, especially from obese mothers, condition neonatal adipogenesis and future obesity risk. His lab has developed several methods to elucidate the cellular and molecular diversity of adipocyte stem-like cells in mice to understand the developmental patterning of fat tissue growth. Through conditional knock-out mice, customized flow cytometry, bulk and single cell RNA-sequencing, and lipid mass spectrometry, Dr. Rudolph is beginning to understand the developmental and cellular cues that control early life patterning of adipose tissue via its resident stem-like cells. This includes the potential for differentiation and subtype heterogeneity, particularly during postnatal fat deposition, when metabolic programming is greatly influenced by infant dietary nutrients. Dr. Rudolph has also established several clinical collaborations to leverage ongoing studies of maternal obesity, human infant adipogenesis, milk fat quality, and composition of maternal fatty acid intake to bridge basic and clinical research. In addition, owing to specific expertise in use of global gene expression analyses, he has applied these skills with other collaborators investigating diverse obesity settings, including human exercise interventions, fetal growth restriction in sheep, hematopoietic stem cells in Macaques, and weight loss maintenance in mice and rats. We have worked extensively with multiple researchers at the University of Colorado to develop and apply the software tools and analytical methods needed to quantify meaningful gene expression changes using high resolution RNA sequencing. As the Associate Director of the NORC MCA Bioinformatics Core, my mission is to support and disseminate the utility of high-throughput genomic technologies to basic, translational, and clinical researchers so they can select the right study design, technology, and methodology that ensures their success.
Molecular Core
Ken Jones, P.h.D.
Associate Director
Coming Soon.
Molecular Core
Matt Jackman, P.h.D.
Acting Manager
Dr. Jackman is a Senior Instructor in the Division of Endocrinology, Diabetes, and Metabolism at the University of Colorado Anschutz Medical Campus. Prior to working at the University of Colorado Denver, my research background was focused on mitochondrial bioenergetics in hepatic and skeletal muscle mitochondria. More recent studies have been focused on isolated skeletal muscle mitochondrial respiration studies in obesity prone (OP) and obesity resistant (OR) rats and mice, and permeabilized human skeletal muscle fibers. Ongoing mitochondrial respiration studies include permeabilized rat/mouse skeletal muscle fibers, osteocytes, and breast cancer cells. In addition to taking over the mitochondrial function sub-core of the MCA, Dr. Jackman has been the Assistant Director of the Anschutz Health and Wellness Animal Satellite facility since 2010.
Molecular Core

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