The Molecular and Cellular Analytical Core has been a service center on the Anschutz campus for over 10 years. 

Core leadership: Jacob (Jed) Friedman, PhD – Director, Rachel Janssen, MS – Manager.

Our Services

  • (Seahorse e96 Analyzer)
  • RNA isolation   
  • cDNA synthesis    
  • Primer design and ordering     
  • Quantitative PCR      
  • ELISA/multiplex assays     
  • Metabolite colorimetric assays    
  • Mitochondrial activity assays

Agilent Seahorse Extracellular Flux Technology

  • The Seahorse XF technology simultaneously interrogates two major energy producing pathways of the cell – mitochondrial respiration and glycolysis – in a microplate, in real-time. The XFe96 Analyzer determines in vitro oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) to assess cellular functions such as oxidative phosphorylation, glycolysis, and fatty acid oxidation. The XFe96 technology provides the tools to rapidly detect cellular responses to substrates, inhibitors and other compounds in a 96-well- plate format.
  •  Initial training on the Seahorse machine is provided. Users are expected to determine their experimental design and interpret their own data. Users will supply their own Seahorse FluxPaks, calibrant, reagents, and assay medium. 
  • Scheduling and training requests can be made via iLab Operations: Molecular and Cellular Analytical Core

Consultation and services for gene expression analysis to investigate a multitude of molecular targets. 

  • RNA isolation from various tissues (liver, muscle, adipose) and cell types (macrophages, cell cultures) from human, nonhuman primate and rodents
  • cDNA synthesis
  •  We currently have over 600 primer sets for human, mouse and rat genes. For primer sets not available in-house, we provide primer design & ordering.
  • Quantitative real-time PCR using SYBR Green technology on genes of interest and two or more reference genes 
  • Please contact Rachel for current prices.

Consultation and services for a broad range of assays over a variety of tissue types, fluids and cells, including cytokine & hormone analyses such as ELISAs and multiplex assays, metabolite colorimetric assays & mitochondrial activity assays. 

  • Please contact Rachel for current prices.

For information or to request services, please contact Rachel Janssen at rachel.janssen@ucdenver.edu